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标题：Structures of ceramide tetrasaccharides from various sources: uniqueness of rat kidney ceramide tetrasaccharide
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作者：Bader Siddiqui ; Jun'ichi Kawanami ; Yu-Teh Li 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1972
卷号：13
期号：5
页码：657-662
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Methylation analysis of ceramide tetrasaccharide isolated from human erythrocytes gave acetates of 2,3,6-tri- O -methylgalactitol and 2,4,6-tri- O -methylgalactitol in a ratio of 1 : 1, and about 50% of the galactose was oxidized by periodate. Rat kidney ceramide tetrasaccharide gave, in contrast, a much larger proportion of the acetates of 2,4,6-tri- O -methylgalactitol (ratio 1 : 0.3), and less than 20% of the galactose was oxidized by periodate. Sequential degradation by β- N -acetylhexosaminidase, α-galactosidase, and β-galactosidase showed ceramide tetrasaccharides to have identical carbohydrate sequences and anomeric structures. The major part of ceramide trihexoside derived from rat kidney ceramide tetrasaccharide migrated on thin-layer chromatography more slowly than that derived from other ceramide tetrasaccharides. The structure of a major part of rat kidney ceramide tetrasaccharide was thus determined to be GalNAcβ(1→3)Galα(1→3)Galβ(1→4)Glcβ-(1→1)Cer, whereas other ceramide tetrasaccharides have Galα(1→4) structure at the penultimate residue.
关键词：human erythrocytes ; monkey kidney ; horse spleen ; bovine kidney ; rat kidney ; methylation ; periodate ; glycosidases ; Galβ(1→3)Gal ; Galα(1→4)Gal