文章基本信息

标题：Identification and quantitation of free ceramides in human platelets
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作者：William Krivit ; Sven Hammarström
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1972
卷号：13
期号：4
页码：525-530
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Free ceramides were isolated from human platelets. Their structures were unequivocally determined by gas–liquid chromatography–mass spectrometry of the trimethylsilyl ether derivatives. The major components were N -(palmitoyl) sphingosine, N -(stearoyl) sphingosine, N -(eicosanoyl) sphingosine, N -(docosanoyl) sphingosine, N -(tetracosanoyl) sphingosine, and N -(tetracosenoyl) sphingosine. Sphinganine- and sphingadienine-containing ceramides as well as ceramides containing other unsaturated acids were also present. The amount of ceramides was determined by quantitative gas–liquid chromatography, using radioactive ceramide as internal standard and synthetic crystalline ceramides for comparison of peak areas. The concentration of ceramides was found to be 1.31 μg/lO⁹ platelets or 0.47 μg/mg of platelet protein.
关键词：gas–liquid chromatography–mass spectrometry ; trimethylsilyl ethers