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标题：Immunochemical studies of organ and tumor lipids. XIX. Cytolytic action of antibodies directed against cytolipin R
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作者：Keizo Inoue ; Liselotte Graf ; Maurice M. Rapport 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1972
卷号：13
期号：1
页码：119-127
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Rabbit antisera to rat lymphosarcoma contain antibodies that are cytolytic for rat erythrocytes in the presence of complement. The reaction can be inhibited completely by pure cytolipin R showing that ( a ) immune hemolysis can be mediated through lipid determinants in the membrane, and ( b ) that cytolipin R determinants are present in the intact erythrocyte membrane and exposed on the surface. Optimal conditions for measurement of cytolysis in this system based on release of ⁵¹Cr are described. Degrees of specificity of a number of different antilymphosarcoma sera are shown, based on inhibition of cytolysis by cytolipin R, cytolipin K, cytolipin H, cytolipin F (F-hapten), glucocerebroside, galactocerebroside, ceramide trisaccharide (cer-glu-gal-gal), and a mixed brain ganglioside preparation. The data suggest that cytolytic antibodies and agglutinating antibodies in these antisera are distinctive despite their common specificity for cytolipin R. Lymphosarcoma cells are more effective than erythrocytes in absorbing cytolytic antibodies.
关键词：haptens ; rat erythrocytes ; hemagglutinin ; ⁵¹Cr ; cytotoxicity ; lymphosarcoma ; membrane