文章基本信息

标题：A method for the determination of lipoprotein lipase in postheparin plasma and body tissues utilizing a triolein-coated Celite substrate
本地全文：下载
作者：Israel Posner ; Virgilio Bosch
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1971
卷号：12
期号：6
页码：768-772
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A simple and specific method for assaying lipoprotein lipase activity is described. Postheparin plasma, heart homogenates, or extracts of acetone powder of adipose tissue were incubated with a triolein-coated Celite substrate, and enzyme activity was determined from the rate of free fatty acid (FFA) release in the incubation system. FFA release was linear for 30 min, and was proportional to protein concentration in the incubation system. FFA release was decreased by addition of deoxycholate or Triton X-100. Increasing the concentration of heparin in the incubation system caused a gradual decrease in FFA release by postheparin plasma and increases in activity of heart homogenates and adipose tissue lipoprotein lipase. The Celite substrate was found to be satisfactory for assaying pancreatic lipase activity as well.
关键词：adipose lipoprotein lipase ; heart lipoprotein lipase