文章基本信息

标题：Lipid patterns in human leukocytes maintained in long-term culture
本地全文：下载
作者：EUGENE L. GOTTFRIED
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1971
卷号：12
期号：5
页码：531-537
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The lipid composition of leukocytes maintained in long-term culture was examined in order to clarify the role of immaturity in previously observed differences between normal mature leukocytes and leukemic cells. Cell cultures derived from three types of leukocytes were examined: normal lymphocytes, Burkitt lymphoma, and chronic myelocytic leukemia. Lipid extracts were analyzed for total lipid weight, phospholipids, neutral lipids, and glycolipids. Distribution of individual phospholipids was determined by quantitative two-dimensional thin-layer chromatography. The main phospholipids were phosphatidylcholine (51–54%) and phosphatidylethanolamine (24–25%), with smaller amounts of phosphatidylinositol, phosphatidylserine, sphingomyelin. and cardiolipin. All three types of cultured cells showed a remarkable similarity in total phospholipid content (17–18 × 10^–15 moles/cell) as well as inphospholipid distribution. More variation was seen in neutral lipid content. Glycolipid was abundant (17–23% of total lipid weight) and was present mostly as ceramide dihexoside. Compared with normal lymphocytes or polymorphonuclear leukocytes, the cultured cells showed increased phosphatidylcholine, decreased sphingomyelin, and decreased cholesterol content, similar to the changes found in leukemic leukocytes. These findings suggest that the altered lipid patterns foundin leukemic leukocytes are a reflection of cell immaturity rather than a characteristic peculiar to the leukemic state.
关键词：tissue culture ; Burkitt lymphoma ; leukemia ; phospholipids ; glycosphingolipids ; cholesterol ; thin-layer chromatography