文章基本信息

标题：A new chromatographic system for vitamin D3, and its metabolites: resolution of a new vitamin D3, metabolite
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作者：M. F. HOLICK ; H. F. DeLUCA
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1971
卷号：12
期号：4
页码：460-465
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A simple yet powerful new chromatographic procedure for vitamin D₃ and its metabolites is described. Liquid–gel partition chromatography on Sephadex LH-20 using a solvent of various percentages of CHCl₃ in Skellysolve B (petroleum ether, bp 67–69°C) permits excellent resolution of vitamin D₃, 25-hydroxyvitamin D₃, and their more polar metabolites. Of special importance is the resolution of the metabolites of vitamin D₃ more polar than 25-hydroxycholecalciferol. Because of this resolution, a new metabolite of vitamin D₃ has been demonstrated in the plasma of rats and in the intestines of chicks given 100 IU of vitamin D₃-1,2-³H.
关键词：Sephadex LH-20 ; cholecalciferol ; intestine ; plasma ; chick ; rat ; 25-hydroxycholecalciferol