文章基本信息

标题：Quantitative isolation of total glycosphingolipids from animal cells
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作者：Terunobu Saito ; Sen-itiroh Hakomori
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1971
卷号：12
期号：2
页码：257-259
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of ( a ) acetylation of total lipids with pyridine and acetic anhydride, ( b ) separation of acetylated glycolipids from nonglycolipids on a magnesia–silica gel (Florisil) column, and ( c ) deacetylation of glycolipid in chloroform–methanol–sodium methoxide. This method is useful for determination of microgram quantities of glycolipids derived from less than 1 ml of packed cells.
关键词：glycolipids ; acetylation ; acetic anhydride ; pyridine ; magnesia–silica gel (Florisil) ; 1,2-dichloroethane (DCE) ; acetone ; deacetylation