文章基本信息

标题：Mechanism of squalene biosynthesis: evidence against the involvement of free nerolidyl pyrophosphate
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作者：S. S. Sofer ; H. C. Rilling
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1969
卷号：10
期号：2
页码：183-187
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Several mechanisms that utilize farnesyl pyrophosphate and nerolidyl pyrophosphate as condensing substrates have been postulated for the asymmetric condensation reaction in squalene biosynthesis. Although there is ample evidence that farnesyl pyrophosphate is a substrate for this reaction, there has been no information concerning the role of nerolidyl pyrophosphate. We have made the following observations that demonstrate that nerolidyl pyrophosphate cannot be a free intermediate in squalene biosynthesis. ( a ) There is no significant interconversion of farnesyl pyrophosphate and nerolidyl pyrophosphate in a squalene-synthesizing system from yeast. ( b ) Nerolidyl-1-³H₂ phosphate is not converted to squalene in the presence or absence of farnesyl pyrophosphate. ( c ) The addition of unlabeled nerolidyl pyrophosphate to incubation mixtures does not alter the relative loss of α-hydrogens from farnesyl pyrophosphate during its conversion to squalene. The synthesis of nerolidyl-1-³H₂ rophosphate is described. Chromatographic methods for the separation of pyrophosphate esters of triprenols and terpenols are included.
关键词：yeast ; farnesyl pyrophosphate ; triprenol and terpenol pyrophosphates ; chromatography ; synthesis of nerolidyl pyrophosphate