文章基本信息

标题：Quantitative glass paper chromatography: a microdetermination of plasma cholesterol
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作者：Joseph R. Swartwout ; Julius W. Dieckert ; O. Neal Miller 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1960
卷号：1
期号：4
页码：281-285
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A rapid, sensitive method for the determination of sterols is described. Microgram samples are spotted on impregnated glass fiber paper, chromatographed for 7 minutes, dried, sprayed with sulfuric acid, and charred by heating. The amount of char formed is measured by densitometry. Values obtained for cholesterol in the serum are in agreement with those obtained by the Sobel-Mayer method. The method has been adapted to the determination of cholesterol in 10 μl. of plasma, and to the determination of standard solutions of other sterols.