文章基本信息

标题：Quantitative glass paper chromatography: phosphatidyl choline and sphingomyelin
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作者：James E. Muldrey ; Neal Miller ; James G. Hamilton 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1959
卷号：1
期号：1
页码：48-52
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A rapid chromatographic procedure was developed for the separation of sphingomyelin, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine, phosphatidyl inositol, and free fatty acids on glass paper coated with sodium silicate. In addition, phosphatidyl choline and sphingomyelin were determined quantitatively by densitometry of the charred chromatogram, which was obtained by spraying the developed chromatogram with sulfuric acid and heating in an oven. The separation of phosphatides on sodium silicate-treated glass paper is more rapid than on silicic acid-impregnated paper, and the former is simpler to prepare. Preliminary application of this quantitative technique to human serum indicates that it may have a wide adaptability for the determination of phospholipids in natural products.