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标题：Expression analysis of new Metallothionein2-like protein under mercury stress in tomato seedling
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作者：Takeshi Nagata
期刊名称：Plant Root
电子版ISSN：1881-6754
出版年度：2014
卷号：8
页码：72-81
DOI：10.3117/plantroot.8.72
出版社：Japan Science and Technology Information Aggregator, Electronic
摘要：Plants including tomato produce several kinds of chelator proteins such as metallothioneins (MTs) for protection against Hg²⁺ toxicity. However, the mechanism of protection from Hg²⁺ is not perfectly clear. Hg²⁺ content subsequently was plateaued from days 1 to 7. Cell death and DNA digestion were not observed in the primary root in the presence of Hg²⁺ over the 7 days. The predicted protein sequences of 5 tomato type 2 MT-like (MT2-like) proteins were compared. The coding sequences of accession number Z68185 had no Cys-Cys motif in the N-terminal. However, the Z68185 cDNA genetic recombinant showed high resistance to Hg²⁺ in bacteria. In tomato, the expression was observed in the roots, but not in the leaves or stems. mRNA of the MT2-like protein was measured in tomato seedlings exposed to 1 μM Hg²⁺. The expression level did not increase until day 3, but increased expression was observed after day 5. These results suggest that new Metallothionein2-like protein express in root specific and it may trap mercury. Our results indicate that functional identification of an MT2-like protein will be useful for molecular breeding designed to improve plant tolerance to Hg²⁺.
关键词：Mercury;Metallothionein2-like protein;Tomato (Solanum lycopersicum);Expression