文章基本信息

标题：Monoclonal Antibody-Based Immunoradiometric Assay for Rat α1-Protease Inhibitor-Bound Anionic Trypsin
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作者：Toshihide KAWAI ; Reiji MORISHITA ; Tadashi EGUCHI 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1990
卷号：9
期号：1
页码：39-50
DOI：10.3164/jcbn.9.39
出版社：The Society for Free Radical Research Japan
摘要：Two monoclonal antibodies that react with epitopes on the α₁-protease inhibitor moiety of rat α₁-protease inhibitor-bound anionic trypsin were generated in mice immunized with the complex. They showed high specificity for the complex in comparison with the free α₁-protease inhibitor. By use of these monoclonal antibodies and another monoclonal antibody that reacts with an epitope on the anionic trypsin moiety of α₁-protease inhibitor-bound anionic trypsin, we established immunoradiometric assays that are specific for the complex. These immunoassays did not give positive results with either free anionic trypsinogen or α₁-protease inhibitor. The immunoradiometric assays were applicable to quantification of α₁-protease inhibitor-bound anionic trypsin in rat sera, and we found that the mean half-life of the complex in the circulation was 140±16.6min (mean±SD)( n =7). Sandwich enzyme-linked immunosorbent assays were also tried. However, they did not give enough specificities to quantify the complex in the rat sera.
关键词：rat α1-protease inhibitor-bound anionic trypsin;monoclonal antibody;immunoradiometric assay