文章基本信息

标题：Improved Procedure for Purification of Human Platelet-Derived Growth Factor
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作者：Kyozo HAYASHI ; Masashi ITOH ; Masayuki KUROBE 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1989
卷号：7
期号：2
页码：81-89
DOI：10.3164/jcbn.7.81
出版社：The Society for Free Radical Research Japan
摘要：A relatively simple method for purification of human platelet-derived growth factor (PDGF) was developed. PDGF was purified from clinically outdated, platelet-rich plasma by means of freezing and thawing extraction and successive chromatography on CM-Sephadex, Sephacryl S-200, and Phenyl Sepharose. Further purification of the PDGF obtained showed two silver-stained bands following polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Amino acid sequence analysis of these two components separated by SDS-PAGE demonstrated that the sequences coincided with those of PDGF A and B chains previously reported and predicted from the nucleotide sequences of the cloned cDNA's of PDGF A and B chain genes. The purified PDGF preparation stimulated a nanogram level of thymidine incorporation into DNA of previously quiescent Balb/c 3T3 cells.
关键词：human platelet-derived growth factor (PDGF);purification;amino acid sequence analysis;[methyl-3H] thymidine incorporation-stimulating activity