文章基本信息

标题：Carbon Tetrachloride-Induced Alteration of Glutathione S-Transferase in Rat Liver Cytosol and Plasma
本地全文：下载
作者：Fumio FUKAI ; Sachiko NISHIZAWA ; Masae KURANO 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1989
卷号：6
期号：3
页码：175-185
DOI：10.3164/jcbn.6.175
出版社：The Society for Free Radical Research Japan
摘要：Effects of carbon tetrachloride (CCl₄) administration on hepatic and plasma glutathione S-transferase (GST) activity were studied in rats under both acute and chronic phases. Intraperitoneal administration of CCl₄ at a high dosage (1.2ml/kg) (acute group) resulted in a marked elevation of plasma GST activity (over 200-fold of the control level at 24h after the administration) that was accompanied by a decrease in hepatic GST activity. In contrast to the successive elevation of plasma GST activity which had not reached a plateau by even 24h, plasma GPT activity reached a maximum at 6h after CCl₄ administration and thereafter decreased to under the control value. Results for substrate specificity of plasma GST suggested that the leakage of hepatic GST into plasma began with by type 1-2 GSTs mainly and continued with not only type 1-2 GSTs but also type 3-4 ones. Supporting this assumption, immunohistochemical analyses of hepatic GSTs showed that the leakage of the GSTs occurred at the centrilobular zone where type 1-2 GSTs were shown to localize dominantly in the normal rat liver. And the leakage developed time-dependently to the surrounding area where both types of GSTs were diffusely distributed. Typical massive cell necrosis also developed in the same region. On the other hand, in the group administered repeatedly with a low dosage (0.4ml/kg) (chronic group), drastic alteration of GST activity and also GST localization could not be observed. These results indicate that elevation of plasma GST activity parallels to quantitative and qualitative alteration of hepatic GSTs as well as the extent of the liver damage in the acute phase. Measurement of plasma GST activity may provide useful information to judge the extent of acute liver damage clinically.
关键词：liver;carbon tetrachloride;liver injury;glutathione S-transferase;plasma GSH-transferase