文章基本信息

标题：Measurement of Serum Superoxide Dismutase Activity by Electron Spin Resonance
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作者：Haruo MIYAGAWA ; Toshikazu YOSHIKAWA ; Toru TANIGAWA 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1988
卷号：5
期号：1
页码：1-7
DOI：10.3164/jcbn.5.1
出版社：The Society for Free Radical Research Japan
摘要：We measured serum superoxide dismutase activity by electron spin resonance, using 100mM 5, 5-dimethyl-1-pyrroline-N-oxide (DMPO) as a trap. The addition of superoxide dismutase decreased the height of the DMPO-OOH signal produced by the hypoxanthine-xanthine oxidase system. Serum superoxide dismutase activity was determined from a quantitative line for superoxide dismutase. When bovine Cu, Zn-superoxide dismutase (50, 000U/kg) was injected intravenously into rats, the superoxide dismutase activity decreased almost linearly with a half life of about 6min. This method allowed direct detection of superoxide radicals and new measurement of superoxide dismutase activity.
关键词：ESR;spin trapping;superoxide dismutase;DMPO