期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1987
卷号：3
期号：1
页码：11-16
DOI：10.3164/jcbn.3.11
出版社：The Society for Free Radical Research Japan
摘要：We applied glucokinase (EC 2.7.1.2) from the thermophilic bacterium Bacillus stearothermophilus that we described in a previous paper, to the analysis of glucose in serum and urine, in combination withglucose-6-phosphate dehydrogenase (EC 1.1.1.49). We found the analytical precision, specificity, and sensitivity to be satisfactory. Results obtained with this method correlated well ( r =0.998 for serum and r =0.997 for urine) with those obtained by a currently available method in which hexokinase (EC 2.7.1.1) is used (hexokinase method), and the working solution of the reagent consisting of the enzyme was far more stable than that of the reagent based on the hexokinase method.
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