文章基本信息

标题：A Stable Glucokinase from a Thermophilic Bacterium
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作者：Sachiko KAMEI ; Kosuke TOMITA ; Kazuhiko NAGATA 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1987
卷号：3
期号：1
页码：1-9
DOI：10.3164/jcbn.3.1
出版社：The Society for Free Radical Research Japan
摘要：A glucokinase (EC 2.7.1.2) was purified from Bacillus stearothermophilus , a moderate thermophile. The native glucokinase, M _r about 68, 000, consists of two identical subunits. This enzyme is relatively temperature- and pH-stable, the optimal pH range for the enzymatic activity being within the stable pH range. It selectively catalyzes the phosphorylation of glucose rather than other kinds of hexoses. The K _m values were estimated to be 1×10^-4M for glucose and 0.5×10^-4M for ATP. It shows no ATPase activity. The high stability, high substrate specificity, low K _m values for glucose and ATP, and the lack of ATPase activity make this enzyme advantageous for use in clinical chemical analysis.
关键词：glucokinase;thermophilic bacterium;purification of glucokinase;properties of glucokinase