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标题：Appearance of Cytosolic Albumin-Like Protein in Microsomal Fraction of Isolated Rabbit Heart during Global Ischemia
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作者：Hiroyuki NAGAHAMA ; Hitoshi TAKENAKA ; Masahiko NAGATA 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2000
卷号：28
期号：1
页码：21-30
DOI：10.3164/jcbn.28.21
出版社：The Society for Free Radical Research Japan
摘要：Isolated rabbit hearts were subjected to hypothermic global ischemia for 1h and subsequent to normothermic Langendorff-reperfusion for 40min. Composition of the protein in the sarcoplasmic reticulum-enriched microsomal fraction was analyzed. Ischemia increased, whereas reperfusion decreased, the levels of a 65-kDa protein. The amino acid sequence in the N-terminal region of this 65-kDa protein was E-A-H-K-S-E-I-A-H-X-F-N-D-V-G-E-E, which resembled the antagonist protein (cytosolic Ca²⁺ uptake inhibitor antagonist protein) found in rabbit heart cytosol (Xu and Narayanan (1994) Mol. Cell. Biochem., 132, 7-14). Thus, ischemia may induce cytosolic albumin to associate with the microsomal fraction of cardiac membranes.
关键词：cytosolic albumin;sarcoplasmic reticulum-enriched microsomes;rabbit heart;ischemia-reperfusion;cytosolic Ca2+ uptake inhibitor antagonist protein