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标题：The Effect of Copper Overload on the Sheep Erythrocyte Membrane
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作者：Aldo S. SANSINANEA ; Silvia I. CERONE ; Angel CATALA 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1996
卷号：21
期号：3
页码：183-190
DOI：10.3164/jcbn.21.183
出版社：The Society for Free Radical Research Japan
摘要：The effect of copper overload on the red blood cell (RBC) membrane from experimentally copper-poisoned sheep was evaluated by studying the lipid peroxidation, composition of fatty acids, and protein organization of RBC membranes. Ten ewes were dosed orally with CuSO₄ in order to induce copper toxicity. The level of lipid peroxidation products was examined in RBC ghosts by measuring their thiobarbituric acid reactivity. The measurement of fatty acids was accomplished by gas-liquid chromatography; and the study of membrane proteins, by SDS-PAGE. The results showed increased lipid peroxidation levels and reduced content of 18:1, 18:2, and 18:3 fatty acids in RBC from copper-poisoned sheep. Despite the RBC copper-overload, treated sheep showed a qualitatively normal protein pattern, but the amount of band 3 of membrane proteins increased. We established a relationship between copper overload and alterations in the RBC membrane structure.
关键词：copper-poisoning;sheep;erythrocyte;membrane;oxidative damage