文章基本信息

标题：Plant Phenols Inhibit Superoxide Anion Radical Production by Murine Peritoneal Macrophages
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作者：Krishan Lal KHANDUJA ; Amita KAUL ; Nirmal Kumar GANGULY 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1996
卷号：21
期号：2
页码：113-122
DOI：10.3164/jcbn.21.113
出版社：The Society for Free Radical Research Japan
摘要：Naturally occurring plant phenols, which included ellagic acid (EA), tannic acid (TA), caffeic acid (CA), and ferulic acid (FA), were tested for their superoxide anion radical (SOR)-scavenging activities. SOR was produced by interaction of tumor promoters, i.e. phorbol-12-myristate-13-acetate (PMA) or mezerein, in vitro with murine peritoneal macrophages. The levels of SOR were assessed microscopically by counting the number of formazan-positive (F⁺) cells/250 cells produced due to the reduction of nitroblue-tetrazolium. PMA at a concentration of 0.1μg/1.85×10⁶cells/0.5ml induced maximum formation of SOR both in resident and thioglycollate-elicited cells, whereas mezerein induced the maximum formation of SOR at 10μg/1.85×10⁶cells/0.5ml. All the tested polyphenols were able to inhibit the formation of SOR induced by the tumor promoters to a variable degree. The maximum inhibition by EA of SOR formation induced by PMA or mezerein was at a concentration of 250μM or 125μM, respectively. The polyphenols CA, TA, and FA were more effective in inhibition of SOR stimulation at a concentration of 120 and 60μM with promoters PMA and mezerein, respectively. However, ferulic acid was found to be the most effective inhibitor of SOR formation.
关键词：superoxide anion radical;peritoneal macrophages;plant phenols;tumor promoters