文章基本信息

标题：Immunoblot Analysis of Urinary Fibronectin of Normal and Passive Heymann Nephritis Rats
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作者：Yoshinori NISHIZAWA ; Fumio FUKAI ; Yasuhiro NATORI 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1996
卷号：20
期号：3
页码：231-239
DOI：10.3164/jcbn.20.231
出版社：The Society for Free Radical Research Japan
摘要：Immunoblot analysis for urinary fibronectin (FN) of normal and passive Heymann nephritis (PHN) rats was performed. When 2-5% skim milk or 2-10% bovine serum albumin solution was used as a blocking buffer for the immunoblot analysis, remarkable nonspecific interactions were observed between many urinary proteins and immunoglobulins used as a primary as well as a secondary antibody. Blocking of the transblot membrane with a 2% γ-globulin solution prevented such nonspecific interactions. As a result, two FN fragments, not intact FN, with molecular masses of 55- and 65-kDa were detected in all the normal rat urine samples. In PHN rat urine, intact FN and several FN fragments of 100-200-kDa were evident instead of the 55- and 65-kDa FN fragments. These results indicate that metabolism of FN and excretion of the FN metabolites into urine may be changed during the development of the renal disease.
关键词：fibronectin;fibronectin fragments;immunoblot analysis;urine;Nephritis