文章基本信息

标题：Levels of Ah-Receptor in Excessive Ascorbic Acid-Fed Guinea Pigs Treated with N-Nitrosodiethylamine
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作者：Sanjay KAPUR ; Rashmi SHARMA ; Krishan Lal KHANDUJA 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1996
卷号：20
期号：3
页码：195-201
DOI：10.3164/jcbn.20.195
出版社：The Society for Free Radical Research Japan
摘要：As the nutritional status of an individual is important in the regulation of xenobiotic metabolism brought about by the activation and detoxification enzymes of mixed function oxidase systems, the balance of these reactions ultimately determines the fate of xenobiotic agents and thereby modulates their toxic, mutagenic, and carcinogenic effects. Among the dietary factors vitamin C has attracted special attention because of its non-toxic nature and tumor prophylactic effects on a number of experimentally induced tumors. In this study we evaluated the effect of N-nitrosodiethylamine (NDEA), a potent environmental carcinogen, on the status of the Ah-receptor (involved in the induction of arylhydrocarbon hydroxylase) in guinea pigs fed excessive doses of ascorbic acid. The Ah-receptor was estimated by two methods, hydroxylapatite assay and Sephacryl S-300 column chromatography. We found that in both the assays the level of Ah-receptors was significantly decreased in excessive ascorbic acid-fed guinea pigs treated with NDEA. The hydroxylapatite method, which was more convenient, fast and economical is to be preferred for the assay of the Ah-receptor.
关键词：arylhydrocarbon hydroxylase;cytochrome P-450;N-nitrosodiethylamine;ascorbic acid;Ah-receptor