文章基本信息

标题：Protective Effect of Catecholestrogen on Lipid Hydroperoxide-Induced Injury to Cultured Bovine Aortic Endothelial Cells and on Lipid Peroxidation of Low-Density Lipoprotein
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作者：Sadaaki KOMURA ; Noriko NAGATA ; Nobuko OHISHI 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1995
卷号：19
期号：2
页码：71-77
DOI：10.3164/jcbn.19.71
出版社：The Society for Free Radical Research Japan
摘要：When bovine aortic endothelial cells were incubated in Dulbecco's modified Eagle's medium containing 5% fetal bovine serum and 5nmol/ml (in terms of malondialdehyde) of linoleic acid hydroperoxide, release of lactate dehydrogenase (LDH) from the cells into the medium increased over a 2-4h incubation period, reaching a maximum level of about 80% of the total activity of LDH. In the presence of 17β-estradiol (E₂) or 2-hydroxyestradiol (2-OHE₂), the LDH release was inhibited depending on their concentration. The IC₅₀ values of the inhibition were approximately 5 and 1μM, respectively. The lipid peroxide level in the cultured endothelial cells increased upon incubation with the hydroperoxide, which increase was suppressed markedly by 2-OHE₂ and slightly by E₂. Lipid peroxidation of low-density lipoprotein was inhibited markedly by 2-OHE₂ and slightly by E₂.
关键词：catecholestrogen;endothelial cells;injury;low-density lipoprotein;lipid peroxidation