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标题：Free-Radical Generation in Regional Ischemic Myocardium Assessed by Electron Paramagnetic Resonance Spectroscopy and Chemiluminescence in Reperfused Canine Heart
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作者：Tsunehiko KUZUYA ; Shiro HOSHIDA ; Youngjoon KIM 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1994
卷号：16
期号：1
页码：1-13
DOI：10.3164/jcbn.16.1
出版社：The Society for Free Radical Research Japan
摘要：The aim of this study was to clarify the role of free radicals generated in the postischemic myocardium in a canine coronary occlusion (90min)-reperfusion (5h) model of myocardial infarction. Myocardial samples were taken from the central infarcted region (infarct), the noninfarcted region within the area at risk (risk), and the endocardial-to-epicardial border between the infarct region and the area at risk (border). Free-radical generation was assessed by electron paramagnetic resonance (EPR) spectroscopy using a spin trapping agent, 5, 5-dimethyl-1-pyrroline N-oxide (DMPO) and a luminol-enhanced chemiluminescence assay. DMPO-adducts detected by EPR spectroscopy consisted of DMPO-OH, which markedly increased after reperfusion in the ischemic myocardium, especially in the border, and showed a peak after 3h of reperfusion ( p <0.01). Similarly, a marked increase in the chemiluminescence of the border was observed after reperfusion, which showed a peak after 3h of reperfusion ( p <0.01) and thereafter declined. The generating activity of free radicals in the border increased significantly ( p <0.01) after reperfusion and its peak was also observed at 3h after reperfusion. However, the activity in the infarct was nearly zero after 1h of reperfusion. Administration of a free-radical scavenger, CV-3611 (5mg/kg, i.v.), before reperfusion reduced the free radical generation in the border. These results indicate that the increased generation of free radicals at the peri-infarcted site may relate to the extension of reperfusion-associated myocardial injury.
关键词：reperfusion injury;free radicals;spin trapping;chemiluminescence;free-radical scavenger