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标题：Formation of 8-Hydroxydeoxyguanosine from Deoxyguanosine by Fe2+/Ascorbic Acid/EDTA/H2O2 System
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作者：Yoshihiro YOSHIMURA ; Yumi MATSUZAKI ; Katsumi UCHIYAMA 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1993
卷号：15
期号：3
页码：155-162
DOI：10.3164/jcbn.15.155
出版社：The Society for Free Radical Research Japan
摘要：8-Hydroxydeoxyguanosine (8-OH-dG) was formed in deoxyguanosine (dG) solution by the addition of Fe²⁺, ascorbic acid (AscA), EDTA, and H₂O₂, and was then detected by ECD after separation by HPLC. 8-OH-dG was not formed in significant amounts when either Fe²⁺, AscA, or H₂O₂ was added separately, or when two of these were added in combination. When Fe²⁺, AscA, and H₂O₂ were added simultaneously, however, 0.87nmol of 8-OH-dG was formed from 2.5μmol of dG. And the addition of EDTA (in the same concentration as Fe²⁺) to the Fe²⁺/AscA/H₂O₂ system caused a remarkable increase in the amount of 8-OH-dG formed (up to 2.89nmol from 2.5μmol of dG). The amount of 8-OH-dG formed increased as the concentration of AscA increased up to 1mM (the same concentration as Fe²⁺). However, higher concentrations of AscA inhibited the formation of 8-OH-dG.
关键词：iron complex,;hydroxyl radical;Fenton reaction;ascorbic acid