文章基本信息

标题：Cytotoxicity of Human β- and γ-Interferon Produced Simultaneously in Glioma Cells Transfected with Interferon Genes
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作者：Kunio YAGI ; Masaaki MIZUNO ; Jun YOSHIDA 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1992
卷号：13
期号：1
页码：1-6
DOI：10.3164/jcbn.13.1
出版社：The Society for Free Radical Research Japan
摘要：The human γ-interferon (HuIFN-γ) gene inserted in a eukaryotic expression vector, pSVIFN-γ, was entrapped in liposomes having positive charges on their surface. Liposome-mediated transfection of cultured U251-MG glioma cells with the gene resulted in secretion of HuINF-γ into the medium and growth inhibition of the glioma cells. The growth-inhibitory effect was even more potent than that obtained in the previous experiments with human β-interferon (HuIFN-β) gene inserted in a eukaryotic expression vector, pSV2IFN-β. However, when the cells were transfected with both pSVIFN-γ and pSV2IFN-β simultaneously, the cells disappeared completely, indicating that the effect became cytocidal.
关键词：glioma;human β-interferon;human γ-interferon;gene transfer;liposomes