文章基本信息

标题：Possible Inhibitory Effect of Lipid Peroxides on Acid Lipase Activity via Low-Density Lipoprotein in Human Mononuclear Leukocytes
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作者：Fusako MAEHIRA ; Ikuko MIYAGI ; Shinzoh KOHNO 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1991
卷号：11
期号：2
页码：129-138
DOI：10.3164/jcbn.11.129
出版社：The Society for Free Radical Research Japan
摘要：Acid lipase (AL) activity was measured in mononuclear leukocytes (MNL) isolated from 214 subjects. An inverse relation was observed between the AL activity and serum cholesterol (CHO) with age variation: The age groups above 30, which showed low AL activities in their MNL, exhibited high serum CHO; whereas the 20-to-29 age group, which had the highest AL activity among the age groups, showed the lowest CHO value. Moreover, the hypercholesterolemic group with high apo-B levels showed significantly low AL activity in both sexes when compared with the normolipidemic group. Significant negative correlations were also observed in the hypercholesterolemic group between the AL activity and both CHO and apo-B, and between the AL activity and lipid peroxides (LPO). On the other hand, while the addition of low-density lipoprotein (LDL) from patients with type II hypercholesterolemia to the AL assay system and the culture medium of MNL resulted in the inhibition of the AL activity, the inhibitory effect was prevented by preincubation of LDL with anti apo-B serum. These data suggest a certain regulatory mechanism of LDL, the physiological substrate of AL, on the AL activity; this action possibly occurs through lipid peroxides in LDL.
关键词：acid lipase;low-density lipoprotein;lipid peroxides;mononuclear leukocytes;hypercholesterolemia