文章基本信息

标题：Secretion of Human β-Interferon into the Cystic Fluid of Glioma Transfected with the Interferon Gene
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作者：Jun YOSHIDA ; Masaaki MIZUNO ; Kunio YAGI 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1991
卷号：11
期号：2
页码：123-128
DOI：10.3164/jcbn.11.123
出版社：The Society for Free Radical Research Japan
摘要：Using subcutaneous solid tumor produced by U251-SP human glioma cells, we studied the in vivo transfection of the cells with the human β-interferon (HuIFN-β) gene by means of novel liposomes. Since the tumor usually forms a single or multiple cysts when the tumor becomes over 15mm in diameter, we injected liposomes entrapping the gene into the cyst, and found that HuIFN-β was secreted into the cystic fluid. It reached a level of approximately 450IU/ml 48h after the transfection and remained at the same level for 8 days. The growth of the tumor was completely inhibited when the injection was carried out every second day for 10 days. The tumor did not grow at all for 30 days.
关键词：glioma;cystic fluid;human β-interferon;gene transfer;liposomes