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标题：Method for Simultaneous Determination of Pancreatic Phospholipase A2 and Prophospholipase A2 in Human Sera and Its Clinical Significance
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作者：Atsushi MISAKI ; Akihiro FUNAKOSHI ; Masao KONO 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1991
卷号：11
期号：2
页码：91-100
DOI：10.3164/jcbn.11.91
出版社：The Society for Free Radical Research Japan
摘要：Monoclonal antibodies were developed against human pancreatic prophospholipase A₂ (proPLA₂). They had high affinity for proPLA₂ and small cross reactivities with human pancreatic phospholipase A₂ (PLA₂). By S-Sepharose ion-exchange chromatography combined with radioimmunoassay for proPLA₂ and that for PLA₂ both using monoclonal antibodies, proPLA₂ and PLA₂ in human sera could be separated and measured. In healthy individuals, proPLA₂ proved to be the major form of the enzyme, while two out of three patients with pancreatitis showed a larger proportion of PLA₂, which may be an indication of the severity of the pancreatitis. In patients with pancreatic cancer and chronic renal failure, proPLA₂ remained in larger proportion in spite of the very high radioimmunoassay level of PLA₂. The concentrations of both proPLA₂ and PLA₂ in human sera could be estimated by a combination of the two radioimmunoassay systems, suggesting that this combination radioimmunoassay could be used to more precisely diagnose pancreatitis and other diseases exhibiting high levels of immunoreactive PLA₂ than a single radioimmunoassay system.
关键词：human pancreatic prophospholipase A2;monoclonal antibody;radioimmunoassay;ion-exchange chromatography;human serum