文章基本信息

标题：Production and Characterization of Monoclonal Antibodies against Human Pancreatic Phospholipase A2
本地全文：下载
作者：Atsushi MISAKI ; Misao IDE ; Masao KONO 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1991
卷号：11
期号：2
页码：79-89
DOI：10.3164/jcbn.11.79
出版社：The Society for Free Radical Research Japan
摘要：Five hybridoma cell lines producing monoclonal antibodies against human pancreatic phospholipase A₂ (PLA₂) were established after selection from prepared hybridomas on the basis of the affinity of the antibodies they produced. All the monoclonal antibodies (1008, 1015, 1045, 1085, and 2003) proved to have very high affinity toward human pancreatic PLA₂ ( K _a=1.5×10¹¹-1.2×10¹²M^-1), but to have no cross reactivity with porcine pancreatic PLA₂, snake venom PLA₂, and membrane-associated PLA₂ from human spleen. Cross reactivities with human pancreatic prophospholipase A₂ (proPLA₂) ranged from 4 to 49%. Three of the monoclonal antibodies neutralized the enzyme activity of PLA₂, while the other two had no effect on it. The three neutralizing antibodies had smaller cross reactivities with proPLA₂ than the other two. On radioimmunoassay using these monoclonal antibodies, the PLA₂ contents of sera from patients with acute pancreatitis were above normal in every case. These monoclonal antibodies are expected to be useful tools in the diagnosis of acute pancreatitis.
关键词：human pancreatic phospholipase A2;monoclonal anti-body;radioimmunoassay;cross reactivity;acute pancreatitis