文章基本信息

标题：Detection of Cronobacter sakazakii in Powdered Infant Formula using Real-time Fluorescence Single Primer Isothermal Amplification
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作者：Yingli Chen ; Xiaoyan Ma ; Xunzhe Zhang 等
期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2016
卷号：12
期号：12
页码：688-697
DOI：10.19026/ajfst.12.3330
出版社：MAXWELL Science Publication
摘要：Specific primers and blockers were designed based on Cronobacter sakazakii gene (16SrRNA) and Real-time Fluorescence Single Primer Isothermal Amplification (RF-SPIA) was established to detect C. sakazakii . This method identified that C. sakazakii genes were positive, whereas other non- C. sakazakii genes were negative. The sensitivity of this test for C. sakazakii pure culture reached 1.4×100CFU/mL and that for C. sakazakii genome reached 7.5×10¹fg/mL. The detection limit of RF-SPIA in milk powder artificially contaminated with C. sakazakii could reach 8.1×10¹CFU/mL. These results demonstrate that the RF-SPIA detection method for C. Sakazakii is highly sensitive, strong, specific and convenient.