文章基本信息

标题：Safety and Efficacy Evaluation of Degradation of Aflatoxin B1 by Low-temperature Radio Frequency Plasma
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作者：Lingling Zhao ; Qijing Du ; Shiqing Wang 等
期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2016
卷号：12
期号：7
页码：396-403
DOI：10.19026/ajfst.12.2982
出版社：MAXWELL Science Publication
摘要：The purpose of this study was to explore the application of Low-Temperature Radio Frequency Plasma (LTRFP) in the degradation of Aflatoxin B₁ (AFB₁). After AFB₁ was exposed to LTRFP, the degradation rate was analyzed by HPLC and the acute toxicity of the degradation products was evaluated in Wistar rats and Human Hepatocellular carcinoma cells (HepG2). The results showed that AFB₁ could be effectively degraded by LTRFP and the degradation rate decreased with initial AFB₁ concentration rise. In initial concentration 0.2 mg/L, the degradation rate of AFB₁ reached up to 95.34%; nevertheless, as the AFB₁ concentration increased to 100 mg/L, the degradation rate declined markedly to 29.32%. The degradation product with degradation rate 29.32% was subjected to toxicity evaluation in rats and a LD₅₀ of 29.41 mg/kg.bw with 95% confidence limit 25.15-34.40 mg/kg.bw was recorded, which was much higher than that of untreated AFB₁ (0.5 to 10 mg/kg.bw). Similar results were also found in the HepG2 cells and the acute toxicity of the degradation product was reversely proportional to the degradation rate. It could be concluded that LTRFP was a potential alternative for the detoxification of AFB₁ due to the reduced toxicity of the degradation product.