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标题：pKa of Glu325 in LacY
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作者：Natalia Grytsyk ; Junichi Sugihara ; H. Ronald Kaback 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：7
页码：1530-1535
DOI：10.1073/pnas.1621431114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Lactose permease (LacY), a paradigm for the largest family of membrane transport proteins, catalyzes the coupled translocation of a galactoside and a H⁺ across the cytoplasmic membrane of Escherichia coli (galactoside/H⁺ symport). One of the most important aspects of the mechanism is the relationship between protonation and binding of the cargo galactopyranoside. In this regard, it has been shown that protonation is required for binding. Furthermore when galactoside affinity is measured as a function of pH, an apparent pK (pK^app) of ∼10.5 is obtained. Strikingly, when Glu325, a residue long known to be involved in coupling between H⁺ and sugar translocation, is replaced with a neutral side chain, the pH effect is abolished, and high-affinity binding is observed until LacY is destabilized at alkaline pH. In this paper, infrared spectroscopy is used to identify Glu325 in situ. Moreover, it is demonstrated that this residue exhibits a pK_a of 10.5 ± 0.1 that is insensitive to the presence of galactopyranoside. Thus, it is apparent that protonation of Glu325 specifically is required for effective sugar binding to LacY.
关键词：transport ; membrane proteins ; lactose permease ; protonation ; surface-enhanced infrared spectroscopy