文章基本信息

标题：Structural insights into the functional cycle of the ATPase module of the 26S proteasome
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作者：Marc Wehmer ; Till Rudack ; Florian Beck 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：6
页码：1305-1310
DOI：10.1073/pnas.1621129114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In eukaryotic cells, the ubiquitin–proteasome system (UPS) is responsible for the regulated degradation of intracellular proteins. The 26S holocomplex comprises the core particle (CP), where proteolysis takes place, and one or two regulatory particles (RPs). The base of the RP is formed by a heterohexameric AAA⁺ ATPase module, which unfolds and translocates substrates into the CP. Applying single-particle cryo-electron microscopy (cryo-EM) and image classification to samples in the presence of different nucleotides and nucleotide analogs, we were able to observe four distinct conformational states (s1 to s4). The resolution of the four conformers allowed for the construction of atomic models of the AAA⁺ ATPase module as it progresses through the functional cycle. In a hitherto unobserved state (s4), the gate controlling access to the CP is open. The structures described in this study allow us to put forward a model for the 26S functional cycle driven by ATP hydrolysis.
关键词：26S proteasome ; cryo-electron microscopy ; AAA⁺ ATPase ; integrative modeling ; single-particle analysis