文章基本信息

标题：In situ structure of trypanosomal ATP synthase dimer reveals a unique arrangement of catalytic subunits
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作者：Alexander W. Mühleip ; Caroline E. Dewar ; Achim Schnaufer 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：5
页码：992-997
DOI：10.1073/pnas.1612386114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We used electron cryotomography and subtomogram averaging to determine the in situ structures of mitochondrial ATP synthase dimers from two organisms belonging to the phylum euglenozoa: Trypanosoma brucei , a lethal human parasite, and Euglena gracilis, a photosynthetic protist. At a resolution of 32.5 Å and 27.5 Å, respectively, the two structures clearly exhibit a noncanonical F₁ head, in which the catalytic (αβ)₃ assembly forms a triangular pyramid rather than the pseudo-sixfold ring arrangement typical of all other ATP synthases investigated so far. Fitting of known X-ray structures reveals that this unusual geometry results from a phylum-specific cleavage of the α subunit, in which the C-terminal α_C fragments are displaced by ∼20 Å and rotated by ∼30° from their expected positions. In this location, the α_C fragment is unable to form the conserved catalytic interface that was thought to be essential for ATP synthesis, and cannot convert γ-subunit rotation into the conformational changes implicit in rotary catalysis. The new arrangement of catalytic subunits suggests that the mechanism of ATP generation by rotary ATPases is less strictly conserved than has been generally assumed. The ATP synthases of these organisms present a unique model system for discerning the individual contributions of the α and β subunits to the fundamental process of ATP synthesis.
关键词：mitochondrial ATP synthase ; electron cryotomography ; subtomogram averaging ; trypanosome ; rotary catalysis