文章基本信息

标题：Development of Rapid Screening of Campylobacter spp. and the GyrA Mutants Using PCR-DNA Strip and Real-Time PCR
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作者：Chiaki KOJIMA ; Michiru KISHIMOTO ; Machiko MIYATA 等
期刊名称：日本食品微生物学会雑誌
印刷版ISSN：1340-8267
电子版ISSN：1882-5982
出版年度：2016
卷号：33
期号：1
页码：12-18
出版社：Japanese Society of Food Microbiology
摘要：A method to detect Campylobacter species by culture in a food pathogen enrichment broth followed by tagged cocktail polymerase chain reaction and DNA strip was developed. When C. jejuni was detected, we measured mutation of amino acid position 86 in the GyrA gene, which is involved in quinolone resistance. The quinolone-resistant strains with GyrA mutation and quinolone-sensitive strains were clearly differentiated simply by measuring their T _m values after SYBR green real-time polymerase chain reaction. To detect Campylobacter from the enrichment broth and predict the quinolone resistance, these methods took only 1 hr.