The present study was carried out to analyze water quality of river Ganga and Yamuna during Mass bathing at Allahabad. Water samples were collected from eight selected bathing sites of the Ganga and Yamuna. The physico-chemical parameter such as Alkalinity, Biological oxygen demand, Chloride, Calcium hardness, Electric conductivity, Magnesium hardness, pH, Temperature, Turbidity and Total hardness were increased whereas decline in the level of Dissolve oxygen was observed at selected sites of Ganga and Yamuna. All parameters were highly affected at Daraganj and Ramghat. These sites were used frequently for the pilgrims and hence found highest affected zones. At all sites water samples were observed poor and showing that the Ganga has moderately polluted as compared to the Yamuna. The present study indicated an increase in water pollution levels of the Ganga and Yamuna.