文章基本信息

标题：Determination of Ochratoxin A in Poultry Feeds Available in Rabat area (Morocco) by High Performance Liquid Chromatography
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作者：Sifou A. ; Mahnine N. ; Manyes L. 等
期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2016
卷号：7
期号：6
页码：2229-2234
出版社：University of Mohammed Premier Oujda
摘要：Sixty two (n=62) samples of poultry feeds collected from farms in three different cities of the Rabat area (region) in Morocco were surveyed for the presence of ochratoxin A (OTA). Samples were extracted with an-in house validated method. Influence of several extraction solvents was studied for the method optimization. OTA was then identified and quantified with liquid chromatography (LC) coupled to fluorescence detection. Results showed that methanol gives the best average recovery (81.0±1.5 %). The limit of quantification (LOQ) was 0.15 ng/g. The proposed method was successfully applied to the analysis of samples. OTA was present in 19 (30.6%) out of total analyzed samples. OTA levels varied between 0.24 and 26.8 ng/g. The average levels of OTA were 11.3, 0.8 and 9.4 ng/g in samples from farms in Rabat, Salé and Témara, respectively. No sample exceeded the maximum limit (100 ng/g) recommended by the European legislation for OTA in poultry feeds.
关键词：Contamination; Feed; Ochratoxin A; Poultry; HPLC; Morocco