文章基本信息

标题：Study of In-vitro Micropropagation of a Medicinally Important Plant Adhatoda vasica Nees by Auxillary-bud Culture
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作者：Prerna Soni ; A. N. Bahadur ; V. K. Kanungo 等
期刊名称：Indian Journal of Innovations and Developments
印刷版ISSN：2277-5382
电子版ISSN：2277-5390
出版年度：2012
卷号：1
期号：4
页码：199-202
出版社：Indian Society for Education and Environment
摘要：The experiment was carried out at plant tissue culture laboratory, Department of Biotechnology, E.R.R.Govt Sci. Auto. P.G. College, Bilaspur, Chhattisgarh, during the period of Nov.08 2010 to May 09 2011. An efficient protocol was devised for rapid in vitro propagation through shoot bud culture of Adhatoda vasica . In present investigation the proliferating auxiliary shoot cultures were established on MS medium&Gamborg B5 medium supplemented with different concentrations of BAP, NAA, Kinetin&2, 4-D using nodal explants from the field grown mature healthy plant of Adhatoda vasica after 30 days of inoculation. In cultures raised from nodal explants of Adhatoda vasica maximum number of shoots was produced on MS medium supplemented with 1.0mg\l BAP. These explants developed greater than two shoots per nodes while in all other concentration of kn, NAA&2,4-D developed either two or less than two shoots\explants. Highest frequencies of shoot formation and maximum number of shoots per explants were obtained on MS medium supplemented with 1mg\l IBA.
关键词：Adhatoda vasica, Micropropagation, MS Medium, Nodal Explants, Shoot Bud Culture