文章基本信息

标题：EVALUATION OF DRIED PLASMA SPOT SAMPLES IN THE MOLECULAR QUANTIFICATION OF HIV-1 RNA ON ABBOTT REAL TIME ASSAY.
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作者：Felix Dambula ; Victor Makene ; Joseph Wu 等
期刊名称：African Journal of Science and Research
印刷版ISSN：2306-5877
出版年度：2016
卷号：5
期号：4
页码：92-95
出版社：Research, Science and Technology publishers
摘要：Dry plasma spot (DPS) samples have least been studied as tools for HIV-1 DNA/PCR and viral load on abbott molecular systems in Malawi. This study tested the performance of DPS against the WHO-approved Dry blood spot (DBS) samples in the molecular quantification of HIV-1 RNA. The assay used was the Abbott m2000rt which uses RT-PCR to generate amplified product from the RNA pol integrase genome of HIV-1. From each whole blood sample collected from participants, a DBS was prepared first and then after centrifugation a DPS pair was prepared from the plasma. Upon analysis, this study has demonstrated that DPS are very efficient in providing extractible HIV-1 RNA, through RT-PCR of genomic pol integrase region of HIV-1. Results showed that the DBS/DPS Pearson’s correlation increased with increasing viral load for individual paired samples and for samples that had counts of >100,000 copies/mL R2 was incredibly 1. The overall correlation value for consolidated individual DBS/DPS sample pairs was greater recording R2=0.92. All in all, this study has demonstrated that DPS can perform better as samples for quantifying HIV-1 RNA (pol-integrase region of genome) via RT-PCR
关键词：Dry plasma spots; DBS viral load; abbott m2000rt; HIV-1; Malawi