文章基本信息

标题：IN VIVO AND IN VITRO LIMITED PROTEOLYSIS OF PHASEOLIN: FACTS, SUGGESTIONS AND PROBLEMS
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作者：Angela RUDAKOVA ; Serghei RUDAKOV ; Natalia LAPTEVA 等
期刊名称：Studia Universitatis Moldaviae: Stiinte Sociale
印刷版ISSN：1814-3199
电子版ISSN：2345-1017
出版年度：2007
卷号：1
期号：1
页码：101-111
出版社：Moldova State University
摘要：As a rule, the complete degradation of 11S and 7S seed storage globulins can be achieved under the action of a single endogenous enzyme, namely papain-like proteinase PP [1]. The PP and another endogenous enzyme, an Asn-specific proteinase LP (legumain [2]), are both synthesized during seed germination. Both are responsible for storage globulin degradation, although the LP seems to play only an accessory role in the process. In vivo as well as in vitro the degradation of storage globulins starts with limited proteolysis that con-sists in specific processing-like cleavages of several peptide bonds of enhanced susceptibility [3]. After in vitro exhaustion of these bonds, further degradation of storage globulins by PP follows a cooperative mechanism that occurs as a pseudo-first order reaction and consists in unlimited one-by-one breakdown of substrate mo-lecules [1,4]. It remains unclear whether the cooperative proteolysis starts from the very beginning of the enzyme action in parallel and irrespective of the initial quick limited proteolysis or the latter brings about structural reformation of storage globulins that triggers the cooperative process.