文章基本信息

标题：El estudio de la biodiversidad en la era de la secuenciación masiva
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作者：Ana E. Escalante ; Lev Jardón Barbolla ; Santiago Ramírez-Barahona 等
期刊名称：Revista Mexicana de Biodiversidad
印刷版ISSN：1870-3453
出版年度：2015
卷号：85
期号：4
出版社：Instituto de Biología
摘要：Recent years have witnessed the advent and rapid development of massive sequencing technology, commonlyknown as Next Generation Sequencing (NGS). This technology allows for rapid, massive and inexpensive sequencingof genome regions or entire genomes, making possible genomic studies of non-model organisms and has seen greatprogress in metagenomic studies. The promise of this information-rich era is to expand the molecular approach ofecological and evolutionary studies towards urgent issues related with conservation and management of biologicaldiversity in the face of global change. Among the current NGS technologies, there are fundamental differences thatimpact DNA sequence accuracy, length and range of applications. Key differences among platforms are the procedurefor library preparation (when needed) and the sequencing process itself (e.g., pyrosequencing, synthesis). In thisreview we describe the technical details of commercially available platforms for massive sequencing.
关键词：massively parallel sequencing, next-generation sequencing, genomics, metagenomics.;secuenciación masiva en paralelo, secuenciación de siguiente generación, genómica, metagenómica.