文章基本信息

标题：Almacenamiento en frío del esperma de la trucha endémica Oncorhynchus mykiss nelsoni: una estrategia para la conservación a corto plazo del germoplasma de especies endémicas
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作者：Marisela Aguilar-Juárez ; Gorgonio Ruiz-Campos ; Carmen G. Paniagua-Chávez 等
期刊名称：Revista Mexicana de Biodiversidad
印刷版ISSN：1870-3453
出版年度：2015
卷号：85
期号：1
出版社：Instituto de Biología
摘要：Aiming to determine an appropriate protocol for short-term storage of the sperm of San Pedro Mártir trout,Oncorhynchus mykiss nelsoni, wild fish previously matured in captivity were anesthetized and the semen, obtainedby abdominal pressure, was suspended in Erdhal and Graham extender at 1:3, 1:6 and 1:9 dilution ratios and kept at4° C for 9 days with an undiluted sample as control. The percentages of sperm motility and membrane integrity wereassessed daily. The results indicated that sperm suspended at a dilution of 1:9 can be stored at 4° C with ≥ 80% motilityand ≥ 65% membrane integrity for 7 and 6 days respectively. In addition, the results show that membrane integritydecreased more rapidly than motility, which suggests that it may be used as an early indicator of sperm damage duringshort-term cold storage. The results obtained are important to support future conservation programs of this trout.
关键词：sperm storage, viability, extender solution.;almacenamiento del esperma, viabilidad, solución extender.