文章基本信息

标题：PARTICIPACIÓN DE DIFERENTES GENES EN LA REPARACIÓN DE RUPTURAS DE DOBLE CADENA EN CEPAS DE ESCHERICHIA COLI EXPUESTAS A RADIACIÓN GAMMA
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作者：Jorge Humberto Serment Guerrero ; Estefany Martínez Martínez ; David Alcántara Díaz 等
期刊名称：Revista Internacional de Contaminación Ambiental
印刷版ISSN：0188-4999
出版年度：2012
卷号：29
期号：1
页码：47-57
语种：Spanish
出版社：Centro de Ciencias de la Atmósfera
摘要：All living organisms are naturally exposed to radiation from different sources. Ionizing radiation produces a plethora of lesions upon ADN that can be categorized as single and double strand breaks and base damage. Among them, unrepaired double strand breaks (DSB) have the greatest biological significance, since they are responsible of cell death. In Escherichia coli this kind of lesions are repaired mostly by homologous recombination. In this work the participation of some recombination genes in the repair of DSB is evaluated. E. coli defective strains were exposed to gamma radiation and incubated for different periods in ideal conditions. Both microelectrophoresis and pulse field gel electrophoresis techniques were used to evaluate the kinetics of repair of such lesions, reflecting the importance of each defective gene in the process.
关键词：DSB repair, recombination, gamma radiation;reparación de rupturas dobles, recombinación, radiación gamma