文章基本信息

标题：Novel Approach to the Rapid Differentiation of Common Prunus Allergen Species by PCR Product Melt Analysis
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作者：Gavin Nixon ; Laurie Hall ; Timothy Wilkes 等
期刊名称：Food and Nutrition Sciences
印刷版ISSN：2157-944X
电子版ISSN：2157-9458
出版年度：2016
卷号：07
期号：10
页码：920-926
DOI：10.4236/fns.2016.710091
语种：English
出版社：Scientific Research Publishing
摘要：Food allergies represent a clear threat to the general health and wellbeing of those affected which place increasing pressure on food producers and regulatory authorities. Current analytical techniques typically find difficulties distinguishing between closely related Prunus species which include almond (Prunus dulcis), an EU listed allergenic species. This study describes a proof of principle real-time PCR approach utilising DNA melt analyses that targets the internal transcribed spacer (ITS) sequence to differentiate between a panel of Prunus test species. The method was successfully applied to the characterisation of a commercial paprika sample suspected of having being adulterated with almond, referred to the UK Government Chemist in 2015 in its advisory capacity. Subject to further validation work, the method appears to specifically amplify Prunus species and is capable of discrimination based on the resultant melt profiles. The developed method provides analysts with a simple and broad molecular tool to identify common Prunus species for food authenticity and allergen testing purposes. Initial development work demonstrates a promising approach with the potential to improve discrimination between Prunus species not easily resolved by routine analytical methods.
关键词：Allergens;Melt Analysis;PrunusSpeciation;Real-Time PCR