文章基本信息

标题：Effect of Tween 80 and Acetone on the Secretion, Structure and Antioxidant Activities of Exopolysaccharides from Lentinus tigrinus
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作者：He, Peixin ; Wu, Shuangshuang ; Pan, Lige 等
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2016
卷号：54
期号：3
页码：290-295
DOI：10.17113/ftb.54.03.16.4211
语种：English
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：In this study, the effects of the addition of Tween 80 and acetone on secretion, structure and antioxidant activities of Lentinus tigrinus exopolysaccharides (EPS) were investigated. It was found that Tween 80 and acetone displayed a stimulatory effect on EPS secretion. The EPS obtained by the addition of Tween 80 (EPS-T), acetone (EPS-A) and control (EPS-C) were purified by Sepharose CL-6B gel filtration chromatography and molecular mass of purified fractions was estimated to be 22.1, 137 and 12 kDa, respectively. Monosaccharide composition analysis indicated that EPS-T, EPS-A and EPS-C were mainly composed of glucose and mannose. Congo Red test indicated that EPS-T and EPS-A had a highly ordered conformation of triple helix, while EPS-C had a random coil conformation. Furthermore, EPS-A exhibited higher DPPH scavenging and antiproliferative activities than EPS-C and EPS-T, which might be attributed to the molecular mass.
关键词：exopolysaccharides; Lentinus tigrinus; molecular mass; molecular conformation; antiproliferative activity