文章基本信息

标题：CRISPR-Cas: an adaptive immunity system in prokaryotes
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作者：Eugene V Koonin ; Kira S Makarova
期刊名称：F1000 Biology Reports
电子版ISSN：2051-7599
出版年度：2009
卷号：1
DOI：10.3410/B1-95
语种：English
出版社：Faculty of 1000 Ltd
摘要：Most of the archaea and numerous bacteria possess an elaborate system of adaptive immunity to mobile genetic elements known as the CRISPR (clustered regularly interspaced short palindromic repeats)-associated system (CRISPR-Cas), which consists of arrays of short repeats interspersed with unique DNA spacers and adjacent operons encompassing CRISPR-associated ( cas ) genes with predicted and, in some cases, experimentally validated nuclease, helicase, and polymerase activities. The system functions by integrating fragments of alien DNA between the repeats and employing their transcripts to degrade the DNA of the respective invading elements via an RNA interference-like mechanism. The CRISPR-Cas system is a case of apparent Lamarckian inheritance.