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标题：Biofortified indica rice attains iron and zinc nutrition dietary targets in the field
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作者：Kurniawan R. Trijatmiko ; Conrado Dueñas ; Nikolaos Tsakirpaloglou 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2016
卷号：6
DOI：10.1038/srep19792
出版社：Springer Nature
摘要：More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g⁻¹ iron (Fe) and 16 μg g⁻¹zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g⁻¹Fe and 28 μg g⁻¹Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g⁻¹ in glasshouse settings; in contrast, under field conditions, 4 μg g⁻¹ was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g⁻¹Fe and 45.7 μg g⁻¹Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase ( OsNAS2 ) and soybean ferritin ( SferH-1 ) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds.