文章基本信息

标题：Overexpression of ThVHAc1 and its potential upstream regulator, ThWRKY7, improved plant tolerance of Cadmium stress
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作者：Guiyan Yang ; Chao Wang ; Yucheng Wang 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2016
卷号：6
DOI：10.1038/srep18752
出版社：Springer Nature
摘要：As one of the most toxic heavy metals in the environment, cadmium (Cd) poses a severe threat to plant growth. We previously reported that overexpression of the Tamarix hispida V-ATPase c subunit ( ThVHAc1 ) improved the Cd tolerance of Saccharomyces cerevisiae . In the current study, we further explored the Cd tolerance conferred by ThVHAc1 in Arabidopsis and T. hispida . ThVHAc1 transgenic Arabidopsis had higher seed germination, biomass, and chlorophyll content under CdCl₂ treatment. In Cd-stressed plants, overexpression of ThVHAc1 significantly improved V-ATPase activity and affected the expression of other V-ATPase subunit-encoding genes. Intriguingly, the lower level of ROS accumulation in ThVHAc1- overexpressing lines under CdCl₂ treatment demonstrated that ThVHAc1 may modulate Cd stress tolerance by regulating ROS homeostasis. Transient expression of ThVHAc1 in T. hispida further confirmed these findings. Furthermore, promoter analysis and yeast one-hybrid assay revealed that the transcription factor ThWRKY7 can specifically bind to the WRKY cis -element in the ThVHAc1 promoter . ThWRKY7 exhibited similar expression patterns as ThVHAc1 under CdCl₂ treatment and improved Cd tolerance, suggesting that ThWRKY7 may be an upstream regulatory gene of ThVHAc1. Therefore, our results show that the combination of ThVHAc1 and its upstream regulator could be used to improve Cd stress tolerance in woody plants.