文章基本信息

标题：New insights into functional regulation in MS-based drug profiling
本地全文：下载
作者：Ana Sofia Carvalho ; Henrik Molina ; Rune Matthiesen 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2016
卷号：6
DOI：10.1038/srep18826
出版社：Springer Nature
摘要：We present a novel data analysis strategy which combined with subcellular fractionation and liquid chromatography–mass spectrometry (LC-MS) based proteomics provides a simple and effective workflow for global drug profiling. Five subcellular fractions were obtained by differential centrifugation followed by high resolution LC-MS and complete functional regulation analysis. The methodology combines functional regulation and enrichment analysis into a single visual summary. The workflow enables improved insight into perturbations caused by drugs. We provide a statistical argument to demonstrate that even crude subcellular fractions leads to improved functional characterization. We demonstrate this data analysis strategy on data obtained in a MS-based global drug profiling study. However, this strategy can also be performed on other types of large scale biological data.